AN ULTRA-HIGH PERFORMANCE FOR THE QUANTIFICATION OF VANCOMYCIN

Abstract

Abstract:-A highly sensitive ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method was developed for the quantification of vancomycin (VAN) in low volumes of rabbit serum. For each analysis,2 μL of rabbit serum was precipitated with methanol that contained the internal standard teicoplan in (TEI). The supernatant was transferred into a384 well-plate, diluted with water, overed with a pierce able silicone mat and5 μL was analyzed in positive ionization mode. The UHPLC-MS/MS consisted of an Agilent 1290 Infinity UHPLC system connected to an AB Sciex QTrap®5500 hybrid linear ion-trap triple quadruple mass spectrometer equipped with a Turbo Spray source. chromatographic separation was achieved using a Waters Acquit UPLC BEH C18 (1.7 μm, 2.1 mm × 100 mm) column, a Van-Guard (1.7 μm, 2.1 × 5 mm) guard column and a mobile phase of water and methanol both containing 5 mM ammonium acetate with 0.1% formic acid. Keywords:-Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry, Vancomycin, Rabbit, Serum, Pharmacokinetics